Categories
Microbiology

Complete the ELISA lab in Labster. Be sure to review the Theory behind the topic

Complete the ELISA lab in Labster. Be sure to review the Theory behind the topics within the lab setting to help support your progress through the lab. Take notes on the theory sections to help you write up your lab report after you are done. This lab is heavy on methods, so make sure you take detailed notes there. Take screen shots when you think important images are presented to include in your lab report.
The goal of this lab is to determine the type of cells that produce the most Factor IX to create cheaper medicine for patients with Hemophilia. After the three cell types are discussed, stop for a few minutes to think about what has been presented to you. Create a hypothesis to predict which cell type will produce the most Factor IX.
When you are done with the lab, use the following outline to write a report or presentation. Label each portion of the lab as follows:
Introduction: Start with a broad discussion of Hemophilia and the issues it causes. Narrow your focus down to factor IX. Conclude the introduction with the hypothesis you created while performing the lab. It’s OK if you were not correct.
Methods: Describe how you conducted the lab. Include the specific steps you took to perform the ELISA and analyze the results so someone reading the lab could replicate the experiment.
Results/Data: Describe your results here. Tables and charts would be appropriate. Images from the lab are also good to support your results.
Discussion: Review your results and determine if your experiment supports or refutes your hypothesis. Explain why. Then expand your discussion and address how your results can be applied to Hemophilia. Be specific and give details.
Requirements:
If you are writing the report, it should be 4-5 pages long, not including the required title and references pages. If you are creating the presentation, it should be at least 10 slides long, not including the required title and references slides.
Reference at least two journal articles.
Follow APA formatting. You can find APA sources in the CSU Global Writing Center.
Include a title and references pages/slides.

Categories
Microbiology

Review the diseases of the immune system in the reading from this week. Choose o

Review the diseases of the immune system in the reading from this week. Choose one disease to discuss in detail. Do not repeat diseases. Post the name of the disease in bold at the top of your post. In the body of your post, be sure to include the following:
The symptoms of the immune disease in people
Causes of the symptoms—is something not functioning normally?
Methods to help control the symptoms
Treatment options
Some ideas for response posts include adding more information about the disease by incorporating new material from a resource or comparing the disease to another one of interest to you.
Be sure to post an initial, substantive response by Thursday at 11:59 p.m. MST, and respond to two or more peers with substantive responses by Sunday at 11:59 p.m. MST. A substantive initial post answers the question presented completely and/or asks a thoughtful question pertaining to the topic. It should be at least 200 words in length. Be sure your post is unique. Do not repeat what other students have said. Substantive peer responses ask a thoughtful question pertaining to the topic and/or answer a question (in detail) posted by another student or the instructor.
Note: The following are not examples of substantive contributions:
Thanking a classmate.
Agreeing with a classmate.
Complimenting a classmate.
Providing irrelevant commentary.
Always support your statements with logic and argument, citing any sources referenced. Post your initial response early and check back often to continue the discussion. Be sure to respond to your peers’ and instructor’s posts, as well.

Categories
Microbiology

Create a brief essay describing innate and adaptive immunity, and why these are

Create a brief essay describing innate and adaptive immunity, and why these are not enough to protect people from diseases. Address the following points:
What is innate immunity? Include at least one physical, one chemical, and one cellular defense in your discussion.
What is adaptive immunity? Be sure to discuss the primary and secondary responses, along with specificity and memory.
How do microbes evade our immune system to cause disease? Can the microbe you are using for your Portfolio Project evade the immune system?
What other ways can people protect themselves from disease?
You are not limited to these points, but they should be included in your paper.
Your well-written paper should meet the following requirements:
Be 2-3 pages in length, not including your cover and reference pages.
Be formatted according to the CSU Global Writing Center. (Links to an external site.)
Include a title page and reference page.
Cite a minimum of th

Categories
Microbiology

This week you will be completing the Biosafety lab in Labster. The lab introduce

This week you will be completing the Biosafety lab in Labster. The lab introduces HazMat containment of a powdery envelope and how to work in a Biosafety Level 3 lab to identify the powder. Instead of writing up a lab report for this lab you will be submitting a paper or presentation focused on managing Hazardous Material. After completing the lab, please submit a paper or presentation addressing the following points:
Introduce anthrax, including symptoms associated with exposure and its virulence.
Find an article discussing an incident of anthrax release in public and discuss the procedures taken to contain it, or what should have been done if specific details are not present.
Discuss why scientists work with anthrax in a Biosafety Level 3 Lab.
Review safety procedures for working with anthrax in a Biosafety Level 3 Lab, including laboratory setup, entry and exit procedures, protective gear, and the use of a biosafety cabinet.
Reflect on your experience working in the virtual level 3 lab. Was it cumbersome? Are all the precautions necessary? How important is it to have biosafety labs to identify hazardous microorganisms?
In 2019 the U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID) at Fort Detrick, MD was shut down. Research this incident and discuss pros and cons to shutting the facility down. Was USAMRIID important for maintaining and controlling biosafety within the United States? Was the shut down for valid reasons? Is there information missing about the closure? Did politics play a role? End with the current state of the shutdown and what you think, or hope, will happen next.
Requirements:
If you are writing the report, it should be 3-4 pages long, not including the required title and references pages. If you are creating the presentation, it should be at least 8 slides long, not including the required title and references slides.
Follow APA formatting. You can find APA sources in the CSU Global Writing Center.
Include a title page/slide. Include a references page/slide at the end.

Categories
Microbiology

This week you will be completing the Biosafety lab in Labster. The lab introduce

This week you will be completing the Biosafety lab in Labster. The lab introduces HazMat containment of a powdery envelope and how to work in a Biosafety Level 3 lab to identify the powder. Instead of writing up a lab report for this lab you will be submitting a paper or presentation focused on managing Hazardous Material. After completing the lab, please submit a paper or presentation addressing the following points:
Introduce anthrax, including symptoms associated with exposure and its virulence.
Find an article discussing an incident of anthrax release in public and discuss the procedures taken to contain it, or what should have been done if specific details are not present.
Discuss why scientists work with anthrax in a Biosafety Level 3 Lab.
Review safety procedures for working with anthrax in a Biosafety Level 3 Lab, including laboratory setup, entry and exit procedures, protective gear, and the use of a biosafety cabinet.
Reflect on your experience working in the virtual level 3 lab. Was it cumbersome? Are all the precautions necessary? How important is it to have biosafety labs to identify hazardous microorganisms?
In 2019 the U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID) at Fort Detrick, MD was shut down. Research this incident and discuss pros and cons to shutting the facility down. Was USAMRIID important for maintaining and controlling biosafety within the United States? Was the shut down for valid reasons? Is there information missing about the closure? Did politics play a role? End with the current state of the shutdown and what you think, or hope, will happen next.
Requirements:
If you are writing the report, it should be 3-4 pages long, not including the required title and references pages. If you are creating the presentation, it should be at least 8 slides long, not including the required title and references slides.
Follow APA formatting. You can find APA sources in the CSU Global Writing Center.
Include a title page/slide. Include a references page/slide at the end.

Categories
Microbiology

think of evolutionary relationships and how we use microbial taxonomy.discuss ho

think of evolutionary relationships and how we use microbial taxonomy.discuss how this system is set up and then discuss the benefits of using this system.can you think of any drawbacks of this system?

Categories
Microbiology

Think of evolutionary relationships and how we use microbial taxonomy.discuss ho

Think of evolutionary relationships and how we use microbial taxonomy.discuss how this system is set up and then discuss the benefits of using this system.can you think of any drawbacks of this system?

Categories
Microbiology

In this module you will complete the Molecular Cloning lab in Labster. In this l

In this module you will complete the Molecular Cloning lab in Labster. In this lab you will be observing gene expression following radiation exposure in a living cell using a reporter gene. Be sure to review the Theory behind the topics within the lab setting to help support your progress through the lab. Take notes to help you write up your lab report. You may even want to write part of the report while you are performing the lab. Take screen shots when you think important images are presented to include in your report.
When you are done with the lab, use the following outline either to write a report or create a presentation. Label each portion of the lab as follows:
Introduction: Give background on damage to DNA caused by UV radiation and the issues caused when DNA cannot repair itself properly. Discuss how DNA repairs itself with a specific focus on RAD52. Introduce the importance of monitoring DNA expression to assess functionality of RAD52.
Methods: Include the following explanations:
how you isolated RAD52 and eGFP DNA including DNA extraction, amplification, restriction enzyme digestion, and gel electrophoresis;
assembly of the fusion gene and expression plasmid using DNA ligation;
transformation of the plasmid into yeast cells and the use of antibiotic selection after transformation; and
the UV radiation experiment.
Results: Describe your results here. Images from the lab are also good to support your results if you took some.
Discussion: Review your results and discuss the importance of being able to observe gene expression. Why is this important for studying DNA repair? What implications does this have for cancer patients?
Requirements:
If you are writing the report, it should be 4-5 pages long not including the required title and references pages. If you are creating the presentation, it should be at least 10 slides long not including the required title and references slides.
Reference at least two journal articles.
Follow APA formatting. You can find APA sources in the CSU Global Writing Center.

Categories
Microbiology

Bacterial Isolation This week you will be completing the Bacterial Isolation lab

Bacterial Isolation
This week you will be completing the Bacterial Isolation lab in Labster. This lab has three parts. Your goal is to identify the bacteria causing an infection, trace its origin, and verify the strains. It may be beneficial to complete the sections of the lab report or presentation as you go through the lab. Review the Theory sections to support your report. When you are done, please submit an APA-formatted report or presentation as outlined below.
Part 1: Identify the bacteria
Introduction: Introduce the problem observed in the hospitalized boy. Conclude with a hypothesis about what could be causing the problem.
Methods: Describe how you analyzed the sample from the hospital. Include details on how the agar plate can be created to promote or inhibit growth in certain bacteria, and what was used in your Salmonella Shigella agar plates here.
Results/Data: Describe your results here. What did you find when you viewed the Salmonella Shigellaagar plates? How did you identify the bacteria?
Discussion: Review your results and determine if your experiment supports or refutes your hypothesis. Explain how you identified this as an antibiotic-resistant bacteria and how the boy was treated following diagnosis.
Part 2: Trace the origin of the bacteria
Introduction: Provide background on what led to infection in the boy. Create a hypothesis regarding the cause of infection that can be tested.
Methods: Describe how you gathered and analyzed samples, including the kind of agar plate used and why you needed to streak the samples.
Results/Data: Describe the streaking results here, including how you can identify the different bacteria present.
Discussion: Review your results in relation to your hypothesis and what needs to be done now.
Part 3: Isolating bacteria
Introduction: Introduce the need for a comparative analysis to verify the origin of the bacteria.
Methods: Describe how you isolated a bacterial colony from the sample.
Results/Data: Did you successfully isolate the bacteria?
Discussion: What are the next steps after isolation? How will the results be used?
Requirements:
If you are writing the report, it should be 3-4 pages long, not including the required title and references pages. If you are creating a presentation, it should be at least 8 slides long, not including the required title and references slides.
Follow APA formatting. You can find APA sources in the CSU Global Writing Center.
Your full lab grade will appear within the Part 2 Lab Report grade.

Categories
Microbiology

Bacterial Isolation This week you will be completing the Bacterial Isolation lab

Bacterial Isolation
This week you will be completing the Bacterial Isolation lab in Labster. This lab has three parts. Your goal is to identify the bacteria causing an infection, trace its origin, and verify the strains. It may be beneficial to complete the sections of the lab report or presentation as you go through the lab. Review the Theory sections to support your report. When you are done, please submit an APA-formatted report or presentation as outlined below.
Part 1: Identify the bacteria
Introduction: Introduce the problem observed in the hospitalized boy. Conclude with a hypothesis about what could be causing the problem.
Methods: Describe how you analyzed the sample from the hospital. Include details on how the agar plate can be created to promote or inhibit growth in certain bacteria, and what was used in your Salmonella Shigella agar plates here.
Results/Data: Describe your results here. What did you find when you viewed the Salmonella Shigellaagar plates? How did you identify the bacteria?
Discussion: Review your results and determine if your experiment supports or refutes your hypothesis. Explain how you identified this as an antibiotic-resistant bacteria and how the boy was treated following diagnosis.
Part 2: Trace the origin of the bacteria
Introduction: Provide background on what led to infection in the boy. Create a hypothesis regarding the cause of infection that can be tested.
Methods: Describe how you gathered and analyzed samples, including the kind of agar plate used and why you needed to streak the samples.
Results/Data: Describe the streaking results here, including how you can identify the different bacteria present.
Discussion: Review your results in relation to your hypothesis and what needs to be done now.
Part 3: Isolating bacteria
Introduction: Introduce the need for a comparative analysis to verify the origin of the bacteria.
Methods: Describe how you isolated a bacterial colony from the sample.
Results/Data: Did you successfully isolate the bacteria?
Discussion: What are the next steps after isolation? How will the results be used?
Requirements:
If you are writing the report, it should be 3-4 pages long, not including the required title and references pages. If you are creating a presentation, it should be at least 8 slides long, not including the required title and references slides.
Follow APA formatting. You can find APA sources in the CSU Global Writing Center.
Your full lab grade will appear within the Part 2 Lab Report grade.